T7 Exonuclease (GENE 6) is Necessary for Molecular Recombination of Bacteriophage T7
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چکیده
منابع مشابه
T7 exonuclease (gene 6) is necessary for molecular recombination of bacteriophage T7.
The role of T7-induced exonuclease (gene 6) in molecular recombination was studied by examining the fate of parental DNA during parental-to-progeny recombination. The method used was to compare infections with T7(+), T7am-6-233 (am gene 6), or T7ts6-136 (ts gene 6) under permissive and nonpermissive conditions. CsCl density gradient analysis of replicative DNA indicated that T7 exonuclease is n...
متن کاملT7 gene 6 exonuclease has an RNase H activity.
T7 gene 6 exonuclease has been shown to have an RNase H activity as well as a double-strand specific DNase activity by the following experiments: The RNase H activity coelutes with the DNase activity from DEAE-cellulose, phosphocellulose, hydroxyapatite, and Sephadex G-200 columns. Gene 6 exonuclease specified by a T7 strain with a temperature sensitive mutation in gene 6 has an extremely heat-...
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Natural biological systems are selected by evolution to continue to exist and evolve. Evolution likely gives rise to complicated systems that are difficult to understand and manipulate. Here, we redesign the genome of a natural biological system, bacteriophage T7, in order to specify an engineered surrogate that, if viable, would be easier to study and extend. Our initial design goals were to p...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 1974
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.14.5.1040-1048.1974